TABLE 2.
Substrate analogs of succinyl-CoA:benzylsuccinate CoA-transferase
| CoA acceptor | CoA donor | Thioester formed (% relative amt) | HPLC retention times of CoA-thioesters (min) |
|---|---|---|---|
| Succinate | Benzylsuccinyl-CoA | 100 | 6.0 |
| Methylsuccinate | Benzylsuccinyl-CoA | 82 | 8.2 |
| Maleinate | Benzylsuccinyl-CoA | 28 | 8.0 |
| Benzylsuccinate | Succinyl-CoA | 100 | 14.3 |
| Methylsuccinate | Succinyl-CoA | 58 | 8.2 |
| Benzylmalonate | Succinyl-CoA | 46 | 10.3 |
| Phenylsuccinate | Succinyl-CoA | 6 | 12.0 |
a
CoA-thioester formation from the given compounds was determined after 20 min of incubation with benzylsuccinyl-CoA or succinyl-CoA as CoA donors. The 100% values correspond to 96 μM for formation of succinyl-CoA and 84 μM for formation of benzylsuccinyl-CoA. No CoA-thioesters were formed with benzylsuccinyl-CoA as CoA donor and formate, acetate, propionate, oxalate, malonate, glutarate, or fumarate as acceptors, or with succinyl-CoA as CoA donor and 2-phenylpropionate, 3-phenylpropionate, or 4-phenylbutyrate as acceptors.