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. 2022 Oct 4;11:e80067. doi: 10.7554/eLife.80067

Appendix 1—key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Antibody anti-CG2678#2
(Rabbit polyclonal)
This paper CG2678#2_4P39glyc, raised against Kipferl peptide R171-I190 Anti-Kipferl polyclonal antibody,
available from Brennecke
lab; ChIP (7 µL per IP)
Antibody anti-CG2678 M4
(Mouse monoclonal)
This paper M4 3A6-3E1-E11, raised against Kipferl amino acids M2-K188 Anti-Kipferl monoclonal
WB antibody, available
from Brennecke lab; WB (1:250)
Antibody anti-CG2678 M3
(Mouse monoclonal)
This paper M3 2 C5-3C3, raised against Kipferl amino acids M2-K188 Anti-Kipferl monoclonal
IF antibody, available
from Brennecke lab; IF (1:500)
Antibody anti-Rhino
(Mouse monoclonal)
This paper 6B7-F2, raised against full length denatured Rhino protein Anti-Rhino monoclonal
WB antibody, available
from Brennecke lab; WB (1:10)
Antibody anti-Rhino
(Rabbit polyclonal)
Mohn et al., 2014 Rhino#1_3573gly ChIP (5 µL per IP),
IF (1:1000)
Antibody anti-Deadlock
(Mouse monoclonal)
Andersen et al., 2017 5B5-6D7-3H10 IF (1:100)
Antibody anti-Nxf3 (Mouse monoclonal) ElMaghraby et al., 2019 8E4-F1 IF (1:50)
Antibody Histone H3K9me3
antibody (Rabbit polyclonal)
Active motif ID_source:39161 ChIP (5 µL per IP), IF (1:100)
Antibody Anti-Histone H3
(di methyl K9)
(mouse monoclonal)
Abcam ID_source:mAbcam1220 ChIP (5 µL per ChIP)
Antibody C1A9 HP1 (Su(var)2–5)
(Mouse monoclonal)
DSHB ID_source:C1A9 ChIP (5 µL per IP), IF (1:100)
Antibody ANTI-FLAG(R) M2
(Mouse monoclonal)
Sigma Aldrich ID_source:F1804-1MG ChIP (2 µL per IP), IF (1:2000)
genetic reagent (D. melanogaster) w1118;;; Bloomington stock 3605 w 1118 wildtype, cultivated in
our lab for several years
genetic reagent (D. melanogaster) w;;; Susan Celniker iso1 wildtype
genetic reagent (D. melanogaster) Cog-GAL4; NGT-GAL4; nos-GAL4; Bloomington stock 31777 Referred to as
'maternal triple
driver' (MTD)
Gal4 driver
genetic reagent (D. melanogaster) w;; pW20>w_sh[attP2]/TM3, Sb; Mohn et al., 2014, VDRC-ID 313772 white (CG2759) RNAi line
genetic reagent (D. melanogaster) w;; pW20>rhi_sh[attP2]/TM3, Sb; Mohn et al., 2014, VDRC-ID 313156 Rhino (CG10683) RNAi line
genetic reagent (D. melanogaster) w;; pW20>CG2678_sh2
[attP2]/TM3, Sb;
this paper Kipferl (CG2678) Kipferl RNAi line,
available from VDRC;
sh-oligo sequence:
ctagcagtCTCGAAGG
CTTTCATGCGTAA
tagttatattcaagcata
TTACGCATGAAA
GCCTTCGAGgcg
genetic reagent (D. melanogaster) w;; pGLKD >piwi_sh2
[attP2]/TM3,Sb;
Senti and Brennecke, 2010, VDRC-ID 313199 Piwi (CG6122) RNAi line
genetic reagent (D. melanogaster) w;; CG2678[Δ1]
(dsRed+)/TM3,Sb;
this paper Kipferl (CG2678) Kipferl mutant allele,
available from VDRC;
LB1-RMCEm31
genetic reagent (D. melanogaster) w;; CG2678[Δ2](dsRed+)/TM3,Sb; this paper Kipferl (CG2678) Kipferl mutant allele,
available from VDRC;
LB1-RMCEm21; has
aberrant deregulation
of blood transposon
genetic reagent (D. melanogaster) w;; CG2678[fs1]/TM3,Sb; this paper Kipferl (CG2678) Kipferl mutant allele,
available from VDRC;
LB1-FSm52; indel (–7);
sequence CCTGCGT
CCTGGCCGTGC-------
TTTCCGGTTCAAGT
GGCAAAGCGAGCAGAG
genetic reagent (D. melanogaster) w;; CG2678RMCE[S161-
3xFLAG]/TM3,Sb;
this paper Kipferl (CG2678) Kipferl tagged construct
(RMCE), available from
VDRC; wildtype 3xFLAG
tagged rescue construct
inserted into LB1-RMCEm31
genetic reagent (D. melanogaster) w; prhino >3xFLAG/V5/
Precission/GFP-
Rhino[attP40],rhi[g2m11]/CyO;
CG2678[Δ1](dsRed+),
nxf3[A2-2]/TM3,Sb;
this paper Rhino, Kipferl, Nxf3 mutant allele combination,
available from VDRC;
Nxf3[A2-2] allele
published in
ElMaghraby et al., 2019
genetic reagent (D. melanogaster) w; prhino >3xFLAG/V5/Precission/GFP-Rhino[attP40],rhi[g2m11]/CyO;; this paper Rhino (CG10683) tagged Rhino construct,
available from VDRC
genetic reagent (D. melanogaster) w; prhino >3xFLAG/V5/Precission/GFP-Rhino[attP40],rhi[g2m11]/CyO; CG2678[Δ1](dsRed+)/TM3,Sb; this paper Rhino, Kipferl mutant allele combination,
available from VDRC
genetic reagent (D. melanogaster) w; rhi[18-7]/CyO;; Andersen et al., 2017, VDRC-ID 313488 Rhino (CG10683) mutant allele
genetic reagent (D. melanogaster) w; rhi[g2m11]/CyO;; this paper Rhino (CG10683) Rhino mutant allele,
available from VDRC;
indel –7; seq: ATGTCT
CGCAACCA-------cc-A
ATCTTGGTCTGGTC
GATGCACCGCCTAATG
genetic reagent (D. melanogaster) w;; pUASz >CG2678[attP2]; this paper Kipferl (CG2678) tagged Kipferl construct,
available from VDRC;
intron containing CG2678
sequence including
non-mapped exon3
genetic reagent (D. melanogaster) w;; CG2678RMCE
[ΔZnFarray1-S161-
3xFLAG]/TM3,Sb;
this paper Kipferl (CG2678) tagged Kipferl construct
(RMCE), available from
VDRC; 3xFLAG tagged
ZnF array 1 deletion
construct inserted into
LB1-RMCEm31
genetic reagent (D. melanogaster) w;; CG2678RMCE
[ΔZnFarray2-S161-
3xFLAG]/TM3,Sb;
this paper Kipferl (CG2678) tagged Kipferl construct
(RMCE), available from
VDRC; 3xFLAG tagged
ZnF array 2 deletion
construct inserted into
LB1-RMCEm31
genetic reagent (D. melanogaster) w;; CG2678RMCE
[3xFLAG-ΔZAD]/TM3,Sb;
this paper Kipferl (CG2678) tagged Kipferl construct
(RMCE), available from
VDRC; 3xFLAG tagged
ZAD deletion construct
inserted into LB1-RMCEm31
genetic reagent (D. melanogaster) w;; CG2678RMCE[3xFLAG-ZAD::GCN4]/TM3,Sb; this paper Kipferl (CG2678) tagged Kipferl construct
(RMCE), available from
VDRC; 3xFLAG tagged
ZAD GCN4 replacement
construct inserted into
LB1- RMCEm31
genetic reagent (D. melanogaster) w;; CG2678RMCE
[3xFLAG-ouibZAD]/TM3,Sb;
this paper Kipferl (CG2678) Tagged Kipferl construct
(RMCE), available from VDRC;
3xFLAG tagged ZAD
replacement construct
inserted into LB1-RMCEm31
genetic reagent (D. melanogaster) w;; CG2678RMCE[ΔZnF#4-S161-3xFLAG]/TM3,Sb; this paper Kipferl (CG2678) tagged Kipferl construct
(RMCE), available from
VDRC; 3xFLAG tagged ZnF4
deletion construct inserted
into LB1-RMCEm31
genetic reagent (D. melanogaster) w; prhino >3xFLAG/V5/
Precission/GFP-
Rhino(CSD::GCN4)
[attP40],rhi[g3m13]/CyO;;
this paper Rhino (CG10683) tagged Rhino construct,
available from VDRC;
Rhino indel –14; seq:
TGGGCGTCCCCAGG---------------AGCGGTTTTCCGAA
CGAGAACAACACC,
Rhino CSD was replaced
by the Gcn4 dimerization
domain, homozygous not
viable, crossed to w;
rhi[18-7]/CyO;; for experiments
genetic reagent (D. melanogaster) w; prhino >3xFLAG/
V5/Precission/GFP-
Rhino(art.hinge)
[attP40],rhi[g3m13]/CyO;;
this paper Rhino (CG10683) tagged Rhino construct,
available from VDRC;
Rhino indel –14; seq:
TGGGCGTCCCCAGG---------------
AGCGGTTTTCCGA
ACGAGAACAACACC,
Rhino hinge was replaced
by a scrambled amino acid
sequence of the same length,
homozygous not viable,
crossed to w; rhi[18-7]/CyO;;
for experiments
genetic reagent (D. melanogaster) w;; prhino >3xFLAG/
V5/Precission/GFP-
Su(var)2-5[attP2];
this paper HP1a (CG8409) tagged HP1a construct,
available from VDRC
genetic reagent (D. melanogaster) w, 3xFLAG/V5/
Precission/GFP-HP1b;;;
this paper HP1b (CG7041) Endogenously tagged HP1b,
available from VDRC
genetic reagent (D. melanogaster) w;; 3xFLAG/V5/
Precission/GFP-HP1c;
this paper HP1c (CG6990) Endogenously tagged HP1c,
available from VDRC
genetic reagent (D. melanogaster) w; peggless >TurboID-
linker-vhhGFP-3xHA-
NLS[attP40]/CyO;;
this paper - Transgenic construct,
available from VDRC; TurboID
biotin ligase fused to
GFP nanobody
genetic reagent (D. melanogaster) w;; 3xFLAG/V5/Precission/
GFP(replacing
CG13741 CDS)-NLS
[attP2]/TM3,Sb;
ElMaghraby et al., 2019 Boot (CG13741) tagged construct; nuclear
GFP used as contol
Sequence-based reagent Rhino_g2 This paper CRISRP guide RNA (indel) GACCAAGATTTGGTCGCTGA
Sequence-based reagent Rhino_g3 This paper CRISRP guide RNA (indel) GTCCCCAGGTTCTGGTGAAG
Sequence-based reagent CG2678_g1 This paper CRISRP guide RNA (RMCE left) GTACAAATGATCAGTGCGA
Sequence-based reagent CG2679_g2 This paper CRISRP guide RNA (RMCE right) GAAGGCATTAAGTAGCATG
Sequence-based reagent CG2678_g3 This paper CRISRP guide RNA (indel) GAACCGGAAAGCATTCTGCA
Sequence-based reagent HP1b_g2 This paper CRISRP guide RNA (N-terminal endogenous tagging) CACAATGGCCGAATTCTCAG
Sequence-based reagent HP1c_g2 This paper CRISRP guide RNA (N-terminal endogenous tagging) GATGCGCTCCACCACGAAGT
Strain, strain background (Saccharomyces cerevisiae) Matα, trp1-901, leu2-3,
112, ura3-52, his3-200,
gal4Δ, gal80Δ, GAL2-
ADE2, LYS2::GAL1-HIS3,
met2::GAL7-lacZ
Mohn et al., 2014
Strain, strain background (Saccharomyces cerevisiae) MatA, trp1-901, leu2-3,
112, ura3-52, his3-200,
gal4Δ, gal80Δ, GAL2-
ADE2, LYS2::GAL1-HIS3,
met2::GAL7-lacZ
Mohn et al., 2014
Strain, strain background (Saccharomyces cerevisiae) pOAD Mohn et al., 2014 in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-Rhino Mohn et al., 2014 in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOBD-Rhino Mohn et al., 2014 in YGS1 (Matα)
Strain, strain background (Saccharomyces cerevisiae) pOAD-HP1a Mohn et al., 2014 in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-Rhino(1-100) Mohn et al., 2014 in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-Rhino(101-300) Mohn et al., 2014 in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-Rhino(301-418) Mohn et al., 2014 in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOBD-CG2678-ZF4nolinker this paper Y2H construct, available
from Brennecke lab; in
YGS1 (Matα)
Strain, strain background (Saccharomyces cerevisiae) pOBD-CG2678-190-311 this paper Y2H construct, available
from Brennecke lab; in
YGS1 (Matα)
Strain, strain background (Saccharomyces cerevisiae) pOAD-CG2678FL this paper Y2H construct, available
from Brennecke lab; in
YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-CG2678frag1 this paper Y2H construct, available
from Brennecke lab; in
YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-CG2678frag2 this paper Y2H construct, available from
Brennecke lab; in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-CG2678frag3 this paper Y2H construct, available from
Brennecke lab; in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-CG2678frag4 this paper Y2H construct, available from
Brennecke lab; in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-CG2678frag5 this paper Y2H construct, available from
Brennecke lab; in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-CG2678-ZF1-2 this paper Y2H construct, available from
Brennecke lab; in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-CG2678-ZF1-3 this paper Y2H construct, available from
Brennecke lab; in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-CG2678ZF2-4 this paper Y2H construct, available from
Brennecke lab; in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-CG2678ZF3-4 this paper Y2H construct, available from
Brennecke lab; in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-CG2678ZF4nolinker this paper Y2H construct, available from
Brennecke lab; in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-OuibZAD this paper Y2H construct, available from
Brennecke lab; in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-Rhi19-85 this paper Y2H construct, available from
Brennecke lab; in YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOAD-CG2678-190-311 this paper Y2H construct, available
from Brennecke lab; in
YGS2 (MatA)
Strain, strain background (Saccharomyces cerevisiae) pOBD-CG2678frag1 this paper Y2H construct, available
from Brennecke lab; in
YGS1 (Matα)
Strain, strain background (Saccharomyces cerevisiae) pOAD-HP1CD this paper Y2H construct, available
from Brennecke lab; in
YGS2 (MatA)