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. 2022 Jul 13;135(13):1588–1596. doi: 10.1097/CM9.0000000000002053

Figure 3.

Figure 3

Overexpression of CHCHD2 attenuates MPP+-induced mitochondrial dysfunction and mitochondrial protein loss. (A) Representative images taken by live cell imaging showed obvious alteration in mitochondrial morphology in Flag-Vector and CHCHD2-flag group with or without MPP+ treatment (500 μmol/L, 24 h). (B) The expression of outer mitochondrial membrane protein (Tom20) and mitochondrial matrix protein (Hsp 60) was measured by Western blotting. (C, D) Densitometry analysis of Tom20 and Hsp60 on IBs (mean ± SD; P < 0.05, †P > 0.05). (E) ATP production in different groups was measured in SHSY5Y cells (mean ± SD; ∗P < 0.05, †P > 0.05). ATP: Adenosine triphosphate; CHCHD2: Coiled-coil-helix-coiled-coil-helix domain-containing 2; Hsp60: Heat shock protein 60; IBs: Immunoblots; MPP: Methyl-4-phenylpyridinium; SD: Standard deviation; Tom20: Translocase of the outer membrane 20.