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. 2022 Sep 27;2022:4000617. doi: 10.1155/2022/4000617

Figure 7.

Figure 7

MT1G overexpression could inhibit ferroptosis of ccRCC. (a) QRT-PCR validated the efficiency of MT1G overexpression at transcriptional level in 769-P and CAKI-1 cells (i). Immunoblot assay (ii) and relative densitometric quantification of MT1G proteins (iii) in 769-P and CAKI-1 cells after overexpression of MT1G. (b) Flow cytometry analysis of the cell apoptosis in 769-P and CAKI-1 added erastin. 769-P and CAKI-1 added erastin after overexpression of MT1G for 48 hours (i). Flow cytometry analysis of the cell apoptosis in 769-P and CAKI-1 added sorafenib, and CAKI-1 added sorafenib after overexpression of MT1G for 48 hours (ii). Statistical analysis reveals the apoptotic rate (%) of 769-P and CAKI -1 after overexpression of MT1G (iii). (c) The overexpression of MT1G could recover the influence of erastin and sorafenib on the GSH consumption of 769-P and CAKI-1. (d) The overexpression of MT1G could not influence the iron concentration of 769-P and CAKI-1 added erastin and sorafenib.