FIG. 2.

Western blot of spore extracts probed with anti-GerAA anti-peptide antibody. Conditions used were as for Fig. 1, except that the secondary antibody was used at a 1 in 10,000 dilution. Lanes 1 and 2 contained biotinylated and prestained protein markers, respectively. Arrows by the marker lane indicate the 58 and 40-kDa markers, and the arrow on the right of the gel indicates the GerAA band. Lanes 3 to 5 contained integument, membrane, and soluble fractions, respectively, of dormant spores of strain WB600. Lane 6 contains total extract from WB600 spores, and lane 7 contained an extract of spores of strain AM1422 (gerAΔ gerB null).