FIG. 5.

Western blot of GerAC and GerBC proteins from coat-stripped spores. Each sample track contains ca. 11 μg of protein. Conditions for electrophoresis and Western blotting were as for Fig. 1. Arrows by the marker lane indicate the 58- and 40-kDa markers, and the arrow on the right of the gel indicates the GerAC band. Lane 1, biotinylated markers; lane 2, prestained protein markers; lane 3, total spore extract of strain WB600; lane 4, total spore extract of strain AM047 (gerE36). Lanes 5 and 6 contained total spore extracts of the gerAΔ and gerAΔ gerBΔ strains respectively. Lane 7 contains concentrated extracted coat and outer membrane material from spores of strain AM047, and lane 8 contains protein from the broken, decoated, AM047 spores.