FIG. 6.

Detection of GerAA protein in Western blots of coat-stripped spores. Lanes 3 to 9 contain 11 μg of protein. Conditions were as described for Fig. 2. Lanes 1 and 2 are markers, as in the other Western blots. Arrows by the marker lane indicate the 58- and 40-kDa biotinylated protein standards in Sigma SDS-6B, and the arrow on the right indicates the position of the GerAA band. Lane 3 and 4 contained total spore extracts of WB600 and AM047, respectively. Lanes 5 and 6 contained extracts from the gerA and gerAB null mutant spores, respectively. Lane 7 contains concentrated extracted coat and outer membrane material from spores of strain AM047, and lane 8 contains protein from the broken, decoated, AM047 spores.