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. 2022 Oct 4;13:5856. doi: 10.1038/s41467-022-33516-1

Fig. 5. Transplastomic expression of AMPs and fAMPs as SUMO fusions.

Fig. 5

a Physical maps of constructs for the expression of 6xHis-SUMO fusions under the control of the T7 RNA polymerase promoter (PT7). For information on construct design and the expression elements used, see Figs. 1a and 3a. b Physical maps of constructs for the expression of 6xHis-SUMO fusions under the control of the plastid rRNA operon promoter (Prrn) and the gene10 leader (T7g10L) from phage T7. Note that the transgenes under the control of PT7 have the opposite orientation in the plastid genome in comparison to the Prrn-driven transgenes, to reduce potential read-through from upstream plastid genes. c Seed tests to confirm homoplasmy of transplastomic plants expressing SUMO fusions. Because RAmpER::SUMO-3-Wamdeposin-5 plants were unable to survive on soil, seeds were obtained from a heteroplasmic plant that segregated into dark green (wild-type-like) and pale leaves and branches. Seeds were harvested from pods of the pale branches.