FIG. 2.

(A) Time course of luciferase expression from cells cotransfected with the indicated plasmid (pEGFP-N1 or WT2), or no plasmid (mock) and the luciferase reporter plasmid. Cells were harvested at the indicated time posttransfection and assayed for luciferase activity. The data in RLU were averaged from experiments performed in triplicate. (B) DNA dose-response for the inhibition of reporter gene expression by ExoU. CHO cells were cotransfected with the luciferase reporter plasmid and 2, 4, 8, 16, or 32 ng of effector plasmid (WT2) versus a control vector (pEGFP-N1). After 24 h, cells were harvested and assayed for luciferase activity. The data were averaged from experiments performed in triplicate.