TABLE 1.
Primers used
| Clone | Primer sequencea |
|---|---|
| pCMV-exoU (WT1) | 5′-G CTG AAG CTT ATG CAT ATC CAA TCG TTG GG |
| 5′-CCG AAG CTT GGA TCC TCA TGT GAA CTC CTT | |
| p1-687U-GFP (WT2) | 5′-G CTG AAG CTT ATG CAT ATC CAA TCG TTG GG |
| 5′-CGG TGG ATC CCA TGT GAA CTC CTT ATT CC | |
| p1-633U-GFP (N1) | 5′-G CTG AAG CTT ATG CAT ATC CAA TCG TTG GG |
| 5′-CGG TAA GCT TGG ATC CCG ACG TAA CAG AG | |
| p1-351U-GFP (N2) | 5′-G CTG AAG CTT ATG CAT ATC CAA TCG TTG GG |
| 5′-TC CGG ATC CAC GTT AAT CAT CAC C | |
| p1-123U-GFP (N3) | 5′-G CTG AAG CTT ATG CAT ATC CAA TCG TTG GG |
| 5′-GT AGG ATC CAT TGC TCC CGG GTA TGC C | |
| p22-351U-GFP (N4) | 5′-CTG AAG CTT ATG CAT TCG CAG GCA GCG CA |
| 5′-TC CGG ATC CAC GTT AAT CAT CAC C | |
| p52-351U-GFP (N5) | 5′-CTG AAG CTT ATG CAT TCC GGG AAG TTG CCG |
| 5′-TC CGG ATC CAC GTT AAT CAT CAC C | |
| p100-351U-GFP (N6) | 5′-GCG AAG CTT ATG CAT AGT CGG CCA CCA TTG |
| 5′-TC CGG ATC CAC GTT AAT CAT CAC C | |
| p155-351U-GFP (N7) | 5′-CGC AAG CTT ATG AGC CCG GCG GCG T |
| 5′-TC CGG ATC CAC GTT AAT CAT CAC C | |
| p52-123U-GFP (N9) | 5′-CTG AAG CTT ATG CAT TCC GGG AAG TTG CCG |
| 5′-GT AGG ATC CAT TGC TCC CGG GTA TGC C | |
| p52-155U-GFP (N10) | 5′-CTG AAG CTT ATG CAT TCC GGG AAG TTG CCG |
| 5′-TG CGG ATC CAT ACC TGA GGC CAA AAG G | |
| p52-201U-GFP (N11) | 5′-CTG AAG CTT ATG CAT TCC GGG AAG TTG CCG |
| 5′-TTA CGG ATC CTT GTT GCC CAA GCC CTT T | |
| p52-249U-GFP (N12) | 5′-CTG AAG CTT ATG CAT TCC GGG AAG TTG CCG |
| 5′-AGT AGG ATC CGT CGG CTG GCG TGC | |
| p52-300U-GFP (N13) | 5′-CTG AAG CTT ATG CAT TCC GGG AAG TTG CCG |
| 5′-CGC AGG ATC CGC ATT GAA CAC CAC TAA | |
| p52-578-GFP (N14) | 5′-CTG AAG CTT ATG CAT TCC GGG AAG TTG CCG |
| 5′-ATC TGG ATC CGC ATC GAG TAA CTG CTG | |
| p124-687U-GFP (C1) | 5′-G CTG AAG CTT ATG CAT CTG GCG CTA GAA GAG |
| 5′-CGG TGG ATC CCA TGT GAA CTC CTT ATT CC | |
| p343-687U-GFP (C2) | 5′-TTG AAG CTT ATG CAT CAG GAT GGC GGG GTG |
| 5′-CGG TGG ATC CCA TGT GAA CTC CTT ATT CC | |
| p409-687U-GFP (C3) | 5′-C GTC AAG CTT CTT GAG TTC GAG GGC |
| 5′-CGG TGG ATC CCA TGT GAA CTC CTT ATT CC | |
| p437-687U-GFP (C4) | 5′-GAT AAG CTT GAG CGC GGT GAT TTC AG |
| 5′-CGG TGG ATC CCA TGT GAA CTC CTT ATT CC | |
| p580-687U-GFP (C6) | 5′-ATC AAG CTT ATG CAT ATG CGC GGG CAG ACG GT |
| 5′-CGG TGG ATC CCA TGT GAA CTC CTT ATT CC | |
| p352-687U-GFP (C7) | 5′-CTG AAG CTT ATG CCG GTC CCT GAG ATG |
| 5′-CGG TGG ATC CCA TGT GAA CTC CTT ATT CC | |
| p203-687-GFP (C8) | 5′-TTGA AAG CTT ATG GGC GGC TTC TCT GAG |
| 5′-CGG TGG ATC CCA TGT GAA CTC CTT ATT CC |
a
Letters in bold type correspond to either HindIII or BamHI restriction endonuclease cleavage sites.