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. 2022 Oct 4;13:5842. doi: 10.1038/s41467-022-33488-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A γδ T cells were sorted from human fetal and pediatric thymuses and were subjected to a modified version of 10x genomics 5′ protocol in order to amplify CDR3δ and CDR3γ regions. Flow chart created with BioRender.com. B Frequency (%) of γδ thymocytes out of total CD3⁺ living cells. C Frequency (%) of Vγ9Vδ2 thymocytes out of γδ⁺ thymocytes. R and p values (two-tailed) in B, C were obtained by doing Spearman correlation test in the gestation age graphs, while dot plots were analyzed by two-tailed unpaired t-test. B, C White dots indicate samples used in the sc RNA/TCR-seq experiments. “FT” group: fetal thymus. “PNT” group: post-natal thymus/pediatric thymus. Source data are provided as a Source Data file. See also Supplementary Fig. 1.