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. 2022 Oct 4;13:5805. doi: 10.1038/s41467-022-33110-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a Blood urea nitrogen measurement in healthy WT mice (n = 5) and after nephrotoxic injury (n = 7). b qRT-PCR for YAP1-downstream target CTGF in nephrotoxic injury after CA3 treatment (n = 7). c FACS plots of TC in Pax8/FUCCI2aR mice after nephrotoxic injury, showing diploid (2C), tetraploid (4C) and octaploid or greater (≥8C) TC (n = 8). Colours match the FUCCI2aR reporter. d Percentage of total and ≥8C cycling polyploid TC in Pax8/FUCCI2aR mice after nephrotoxic injury (n = 8). e Total number of FUCCI2aR TC after nephrotoxic injury (n = 8). f PAS staining in WT mice at day 30 after nephrotoxic injury (n = 7). Bars 50 µm. g Senescence-associated β-galactosidase assay in WT mice at day 30 after nephrotoxic injury (n = 7). Bars 100 µm. h Percentage of β-galactosidase⁺ TC (n = 7). i Sequential scanning of kidney sections stained for fibronectin in WT mice at day 30 after nephrotoxic injury (n = 7). Bars 500 µm. DAPI counterstains nuclei. j Quantification of fibronectin deposition by digital morphometry (n = 7). k Blood urea nitrogen measurement in Pax8/WT and Pax8/YAP1ko mice after nephrotoxic injury (n = 8). l FACS plots of TC after nephrotoxic injury showing diploid (2C), tetraploid (4C) and octaploid or greater (≥8C) TC (n = 8). Colours match the FUCCI2aR reporter. m Percentage of total and ≥8C cycling polyploid TC (n = 8). n Percentage of β-galactosidase⁺ TC (n = 8). o Quantification of fibronectin deposition by digital morphometry (n = 8). p Blood urea nitrogen measurement in Pax8/WT mice after nephrotoxic injury (n = 7). q FACS plots of TC in Pax8/FUCCI2aR mice after nephrotoxic injury, showing diploid (2C), tetraploid (4C) and octaploid or greater (≥8C) TC (n = 7). Colours match the FUCCI2aR reporter. r Percentage of total and ≥8C cycling polyploid TC (n = 7). s Percentage of β-galactosidase⁺ TC (n = 7). t Quantification of fibronectin deposition by digital morphometry (n = 7). t30: day 30 after nephrotoxic AKI. t4 recombination: doxycycline administered 4 days after nephrotoxic AKI. Senolytic treatment: quercetin+dasatinib. PAS: Periodic-acid schiff. Statistical significance was calculated by two-sided Mann-Whitney test; numbers on graphs represent exact p values. Data are expressed as mean ± SEM in graph a, k, and p. Box-and-whisker plots: line = median, box = 25–75%, whiskers = outlier (coef. 1.5).