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. 2021 Oct 21;12(10):3986–4003. doi: 10.1016/j.apsb.2021.10.008

Figure 5.

Figure 5

Emodin suppresses pancreatic exosome secretion during SAP. (A) Representative TEM images of exosomes secreted by the pancreas in the sham operation group (NPE), SAP model group (SPE), and emodin treatment group (EPE). (B) Size distribution of pancreatic exosomes in each group, scale bar = 100 nm. (C) Quantitative analysis of pancreatic exosome concentration (n = 6). (D) Western blot analysis of TSG101, CD63, and Calnexin in pancreatic exosomes. β-actin was used as the loading control, and pancreas lysate was used as the control. (E) Quantitative analysis of CD63 and TSG101 expression in pancreatic exosomes (n = 6). (F) Representative TEM pictures of exosomes secreted by acinar cells in the NACE group treated with PBS, MACE group (treatment with 500 μmol/L sodium taurocholate for 1 h), and EACE group (pretreatment with 20 μmol/L emodin for 30 min, followed by treatment with 500 μmol/L sodium taurocholate for 1 h), scale bar = 100 nm. (G) Size distribution of exosomes secreted by acinar cells in each group. (H) Quantitative analysis of exosome production in acinar cells (n = 6). (I) Western blot analysis of TSG101, CD63, and Calnexin in exosomes secreted by acinar cells. β-actin was used as the loading control, and acinar cells lysate was used as the control. (J) Quantitative analysis of CD63 and TSG101 expression in exosomes secreted by acinar cells (n = 6). All experiments were performed three times, independently. ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.001 vs. the NPE or NACE group; #P < 0.05, ####P < 0.0001 vs. the SPE or MACE group, by one-way ANOVA followed by Tukey tests.