FIG. 4.
Effects of PDZ mutations on ςK-directed gene expression when spoIVB is expressed prematurely. β-Galactosidase synthesis was measured at the indicated times following the initiation of sporulation in cells lysogenized with SPβ::gerE-lacZ. Cells carried both spoIIIGΔ1 and bofCΔ::neo, allowing signaling in the ςK checkpoint. In addition, cells carried the following spoIVB alleles at the amyE locus. (A) NH1140, spoIVB+ (●); NH1248, spoIVB− (○); NH1250, spoIVBGA114 (▪); NH1252, spoIVBGA126 (□); NH1254, spoIVBGA144 (▵). (B) NH1140, spoIVB+ (●); NH1248, spoIVB− (○); NH1256, spoIVBND155 (▪); NH1258, spoIVBGA144/ND155 (□); NH1260, spoIVBRK185 (▴); NH1268, spoIVBDN149 (▵). (C) NH1140, spoIVB+ (●); NH1248, spoIVB− (○); NH1262, spoIVBGQ114 (▪); NH1264, spoIVBGQ126 (□); NH1266, spoIVBGQ144 (▵); NH1270, spoIVBNY155 (▴); NH1272, spoIVBRH185 (⋄). Background levels of gerE-directed β-galactosidase synthesis present in cells containing no reporter have been subtracted.