TABLE 1.
Plasmids used in this work
| Plasmid | Description | Reference(s) |
|---|---|---|
| 4H8 | S. coelicolor cosmid, carrying recA region; aphII | 28 |
| pUC18 | bla lacZ | 43 |
| pUC18rec | pUC18, carrying S. lividans recA on a 2,820-bp fragment; bla | Present study |
| pGM8 | Temperature-sensitive pSG5 derivative; tsr aacC1 | 25 |
| pGMhyg | pSG5 derivative carrying the hygromycin phospho-transferase gene hph | 19; Muth, unpublished |
| pSVXS | pUC18 derivative carrying the 3,271-bp XhoI-SalI (partial digest) fragment (recA) of cosmid 4H8 | 28; present study |
| pEXrecA | recA expression plasmid; tsr aacC1 cat recA | 36 |
| pKOrecA | Temperature-sensitive recA replacement vector; hph aphII | Present study |
| pRErecA | Replacement vector for the reconstitution of recA; tsr aacC1 bla | Present study |
| pTWSl1 | pSVB30 derivative carrying the 1,293-bp ApaI-BamHI fragment containing the S. lividans recA gene | Present study |
| pSVB30 | Cloning vector; bla | 2 |
| pIJ920 | SCP2 derivative; vph | 18 |
| pCK3S | pSG5 derivative carrying a 550-bp fragment of snpR; tsr | 24 |
| pJOE2702 | E. coli expression vector, rhamnose induction; bla | 33 |
| pJOE2702lexA | pJOE2702, carrying the S. lividans lexA gene | Present study |