FIGURE 3.

NEDD4L is negatively targeted by miR‐10b‐5p in macrophages. (A) The target site between miR‐10b‐5p and NEDD4L predicted through the online Starbase website. (B) The expression of NEDD4L in brain tissues of 40 cases of glioma and 15 cases of non‐glioma measured by RT‐qPCR. (C) The expression of NEDD4L in brain tissues of 40 cases of glioma and 15 cases of non‐glioma determined by immunohistochemistry (×200). (D) NEDD4L expression negatively correlated with miR‐10b‐5p expression revealed by Pearson correlation analysis. (E) Targeting relation between miR‐10b‐5p and NEDD4L identified by dual‐luciferase reporter gene assay. (F) The expression of miR‐10b‐5p and mRNA expression of NEDD4L in U937 cells after miR‐10b‐5p alteration assessed by RT‐qPCR. (G) Immunoblotting for determination of protein expression of miR‐10b‐5p and NEDD4L in U937 cells. *p < 0.05 versus brain tissues from patients with non‐glioma or NEDD4L 3′UTR‐WT co‐transfection with mimic‐NC. ^# p < 0.05 versus macrophages treated with inhibitor‐NC. The experiment was repeated three times