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. 2022 Sep 2;28(11):1733–1747. doi: 10.1111/cns.13905

FIGURE 4.

FIGURE 4

miR‐10b‐5p stimulates M2 polarization of macrophages to increase proliferation, migration, and invasion of glioma cells via downregulation of NEDD4L. (A) The expression of miR‐10b‐5p and NEDD4L in U937 cells after overexpression of miR‐10b‐5p and NEDD4L measured by RT‐qPCR. (B) The mRNA expression of iNOS, TNF‐α, Arg‐1, and IL‐10 in U937 cells after overexpression of miR‐10b‐5p and NEDD4L determined by RT‐qPCR. (C) The protein expression of iNOS and Arg‐1 in U937 cells after overexpression of miR‐10b‐5p and NEDD4L examined by Immunoblotting. (D) The expression of TNF‐α and IL‐10 assessed in supernatant of U937 cells after overexpression of miR‐10b‐5p and NEDD4L examined by ELISA. (E) The proportion of CD11b+CD163+ cells in U937 cells after overexpression of miR‐10b‐5p and NEDD4L evaluated by flow cytometry. (F) The proliferation in glioma U87 and A172 cells co‐cultured with transfected U937 cells assessed by EdU. (G) Cell migration in U87, and A172 cells co‐cultured with U937 cells tested by Transwell. H, Cell invasion in U87 and A172 cells co‐cultured with U937 cells tested by Transwell. *p < 0.05 versus U937 cells transfected with Vector and mimic‐NC or U87 or A172 co‐cultured with U937 cells treated without anything. # p < 0.05 versus U937 cells transfected with NEDD4L and mimic‐NC or U87 or A172 co‐cultured with U937 cells transfected with NEDD4L and mimic‐NC. The experiment was repeated three times