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. 2022 Sep 2;28(11):1733–1747. doi: 10.1111/cns.13905

FIGURE 7.

FIGURE 7

Macrophage accelerates the formation of glioma in nude mice through hypoxic glioma‐derived EVs carrying miR‐10b‐5p. (A) Images of subcutaneous tumor formation after U937 treated with hypoxic glioma cell‐derived EVs, and U87 cells were transplanted into nude mice (n = 8). (B) Curves of subcutaneous tumor growth in nude mice transplanted with U937 cells treated with hypoxic glioma cell‐derived EVs and U87 cells (n = 8). (C) Tumor weight of tumor in nude mice transplanted with U937 cells treated with hypoxic glioma cell‐derived EVs and U87 cells presented by histogram (n = 8). (D) RT‐qPCR for determination of mRNA expression of miR‐10b‐5p, NEDD4L, PIK3CA (n = 8). (E) The protein expression of NEDD4L, PIK3CA, and AKT and phosphorylated AKT in tumor of nude mice transplanted with U937 cells treated with hypoxic glioma cell‐derived EVs and U87 cells determined by Immunoblotting (n = 8). (F) The expression of Ki67 in tumor of nude mice transplanted with U937 cells treated with hypoxic glioma cell‐derived EVs and U87 cells examined by Immunohistochemistry (n = 8) (×200). *p < 0.05 versus tumors of nude mice transplanted with U87 cells and U937 treated with PBS. # p < 0.05 versus tumors of nude mice transplanted with U87 cells and U937 treated with H‐EV‐mimic‐NC