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. 2022 Jul 20;28(11):1748–1766. doi: 10.1111/cns.13913

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© 2022 The Authors. CNS Neuroscience & Therapeutics published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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U251‐derived PTX3 mediated the migration and polarization of HMC3. (A) Western blotting results of PTX3 expression in NC and siRNA groups. (B) qPCR results of PTX3 expression in NC and siRNA groups. (C) Study design of the coculture system between HMC3 and U251 cells for transwell assay. (D) Representative images of transwell assay for migration of HMC3 in NC and siRNA groups in different time points. (E) Statistical analysis of transwell assay. (F) Study design of the coculture system between HMC3 and U251 cells for flow cytometry assay. (G) Flow cytometry assay results of CD68 and CD163 expression in NC and siRNA groups. (H) Statistical analysis of flow cytometry assay.