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. 2022 Oct 5;12(10):220213. doi: 10.1098/rsob.220213

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© 2022 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — Ionizing radiation evokes cellular NAMPT activity. (a,c) HOK and HUVEC cells were treated with 10 Gy ionizing radiation, and cellular enzymatic activity of NAMPT was measured at indicated time after irradiation (a). The expression of NAMPT was examined by immunoblot (c). IR, ionizing radiation. **p < 0.01. (b,d) HOK and HUVEC cells were treated with ionizing radiation at indicated doses, and cellular enzymatic activity of NAMPT was measured 30 min after irradiation (b). The expression of NAMPT was examined by immunoblot (d). **p < 0.01; ***p < 0.001. (e) HOK cells were treated with ionizing radiation at 10 Gy (left panel) or indicated doses (right panel). The expression of NAMPT was examined by RT-PCR. (f) HOK and HUVEC cells with expression of Flag-NAMPT were treated with 10 Gy ionizing radiation, and Flag-NAMPT protein was precipitated. NAMPT activity in the precipitates was measured. ***p < 0.001.