TABLE 1.
Selection of PCP affinity mutants
| Strain | Selective mediuma
|
Mutant phenotype | No. of independent selectionsb | No. of analyzed mutantsb | No. of plasmidic mutationsb | |
|---|---|---|---|---|---|---|
| Competing substrate (concn, μg · ml−1) | Limiting substrate (concn, μg · ml−1) | |||||
| RW123(pPZ1-7) | CYT (400) | ADE (3) | CYTr | 191 | 183 | 8 |
| 5MeC (200) | ADE (3) | 5MeCr | 50 | 65 | 0 | |
| RW126(pPZ1-7) | ADE (100) | CYT (3) | ADEr | 215 | 89 | 0 |
| HYP (100) | CYT (3) | HYPr | 145 | 73 | 0 | |
| GUA (60) | CYT (3) | GUAr | 110 | 14 | 0 | |
| 5MeC (200) | CYT (3) | 5MeCr | 110 | 81 | 50 | |
| Total | 821 | 505 | 58 | |||
a
Compositions of YNB selective media and phenotypes of selected mutants.
b
Number of isolated mutants and characterization of fcy2 mutations.