Summary of produced IgG antibody conjugates and their evaluation in western blot and direct immunofluorescence experiments.
| Entry | Antigen | Antibody Typea | Tagb | Western Blotc | Direct Immunofluorescencec |
|---|---|---|---|---|---|
| 1 | GAPDH | IgG1 (m, mc) | SiR | Y | Y |
| 2 | GAPDH | IgG1 (m, mc) | BFL | Y | Y |
| 3 | GAPDH | IgG1 (m, mc) | Biotin | Y | Y |
| 4 | GAPDH | IgG1 (m, mc) | PB | —d | —d |
| 5 | β-Actin | IgG1 (m, mc) | SiR | Y | Y |
| 6 | Nav1.5 | —e | BFL | N | Y |
| 7 | Cav1.2 | IgGf (r, pc) | SiR | —g | Y |
| 8 | Na+/K+-ATPase | IgG1 (m, mc) | SiR | N | Y |
| 9 | TRPM4 | IgGf (r, pc) | SiR | Y | Nh |
a
m, mouse; r, rabbit; mc, monoclonal; pc, polyclonal.
b
SiR, Si-rhodamine; BFL, BODIPY FL, PB, Pacific Blue.
c
Y, specific detection or staining of antigen; N, no detection or staining of antigen.
d
Suitable detection system was not available.
e
No information available from supplier.
f
Subtype(s) not known.
g
Western blot not attempted with SiR-modified antibody, as native antibody did not give signal.
h
Native antibody did also not give specific staining in indirect immunofluorescence with secondary fluorescent antibody. Experiments in mouse heart sections showed similar inconclusive results for both SiR-modified and native anti TRPM4 antibodies.