Fig. 4. Typical NIR-II polymethine fluorophores for dynamic and multiplexed imaging. (A) The hydrophobic fluorophore 5H5 was transformed into 5H5-PEG8-cRGDfk through PEGylation which was used for in vivo NIR-II or NIR-IIa tumor targeted imaging. Reproduced with permission from ref. 35. Copyright 2019 American Chemical Society. (B) The water-soluble fluorophore LZ1105 was employed for dynamic tracking and imaging of the carotid artery, and the dynamic process of thrombolysis after injection of thrombolytics was distinctly visualized (yellow arrows). Reproduced with permission from ref. 34. Copyright 2020, Springer Nature. (C) HC1222/F5-TPB (λex = 1120 nm) and HC1342/F5-TPB (λex = 1319 nm) were employed to perform the dual-color imaging in living mice. Reproduced with permission from ref. 39. Copyright 2022 Wiley-VCH. (D) MeOFlav7 (λex = 980) and JuloFlav7 (λex = 1064 nm) combining with ICG (λex = 785) were used for three-colour real time in vivo imaging. Reproduced with permission from ref. 25. Copyright 2020, Springer Nature. (E) Combining ICG (λex = 785 nm), JuloChrom5 (λex = 892 nm), Chrom7 (λex = 968 nm), and JuloFlav7 (λex = 1065 nm) can realize the four-color video-rate imaging in mice. Reproduced with permission from ref. 68. Copyright 2022 American Chemical Society.
