Fig. 2. Self-healing behavior of chemically fueled Fmoc-AAD-OH in a pseudo-steady-state. (A) Confocal micrographs of damaged Fmoc-AAD-OH fibers at 5 minutes with collapsing width over the time of 25 minutes. For each scratch, a triplicate of widths was analyzed over the whole scratch size (each width being in a distance of roughly 40 μm from each other). (B) The width of a scratch as a function of time after damaging at 5 minutes (black) and 30 minutes (light blue). (C) Normalized self-healing behavior as a function of time after damaging at 5 minutes (black) and 30 minutes (light blue). Error bars represent 3 widths per scratch for an average of 3 scratches. (D) Migration rate of fiber assemblies over time of damage after fuel addition. Error bars represent 3 widths per scratch for an average of 3 scratches. (E) Migration rate over added EDU concentration, damaging at 13 minutes after fuel addition. Error bars represent 3 widths per scratch for an average of 3 scratches. (F) Vial inversion pictures of 10 mM Fmoc-AAD-OH without fuel (I), after the addition of 500 mM fuel (II), after vortex mixing 5 minutes after fuel addition (III), relaxing for 5 minutes and inverting (IV). (G) Storage and loss modulus over time of 10 mM Fmoc-AAD-OH fueled with 500 mM EDC. Error bars represent the average of 3 experiments. (H) Recovery of storage modulus of Fmoc-AAD-OH over different damage time after fuel addition. Error bars represent the average of 3 experiments.