Figure 3. Expression of leptin receptors in the ovaries of 21 days-old piglets. For immunostaining, ovarian sections were incubated with leptin receptor antibody (Ob-R (M-18)-R, sc-1834-R; Santa Cruz Biotechnology Inc, Heidelberg, Germany; dilution 1/250), without (A) and with (B) displacement by the peptide used as immunogen for antibody preparation. Leptin receptors are detectable in all germ cells and some granulosa cells of primary follicles. Dotted lines delineate germ cell cysts. Primordial and primary follicles are indicated by black and open arrows, respectively. Bar = 50µm. (Monniaux and Brisard, 2018; INRA, Nouzilly, France; unpublished pictures).
