|
| Antibodies |
| Guinea Pig anti-Perilipin antibody |
Fitzgerald |
Cat#20R-PP004; RRID: AB_1288416 |
| Chicken anti-GFP antibody |
Abcam |
Cat# ab13970; RRID: AB_300798 |
| Mouse anti- α-SMA antibody |
eBioscience™ |
Cat# 14-9760-80; RRID:AB_2572995 |
| Rabbit anti-CD31 antibody |
Abcam |
Cat# ab28364; RRID:AB_726362 |
| Rat anti-Mac2 antibody |
CEDARLANE |
Cat# CL8942AP; RRID:AB_10060357 |
| Rabbit anti-FSP1 antibody |
Abcam |
Cat# ab41532; RRID:AB_945346 |
| Rabbit anti- Desmin antibody |
Cell Signaling |
Cat# 5332; RRID:AB_1903947 |
|
| Biological samples |
|
| Human breast cancer sample slices |
National Disease Research Interchange |
https://ndriresource.org/
|
|
| Chemicals, peptides, and recombinant proteins |
|
| Collagen hybridizing peptide |
Advanced BioMatrix |
Cat# 5276 |
| Collagenase D |
Roche |
Cat# 11088882001 |
| Collagenase B |
Roche |
Cat# 11088831001 |
| Deoxyribonuclease |
Sigma-Aldrich |
Cat# DN25 |
| Intralipid |
Sigma-Aldrich |
Cat# I141 |
| BSA |
Sigma-Aldrich |
Cat# A8806 |
|
| Critical commercial assays |
|
| Seahorse XFe24 FluxPak |
Agilent |
Cat# 102340-100 |
| Mouse Insulin ELISA Jumbo Pack |
ALPCO |
Cat# 80-INSMS-E10 |
| Mouse Leptin ELISA Kit |
Crystal Chem |
Cat# 90030 |
| Mouse Adiponectin ELISA |
EMD Millipore |
Cat# EZMADP-60K |
| LabAssay ™ NEFA |
Wako |
Cat# 633-52001 |
| LabAssay ™ Triglyceride |
Wako |
Cat# 632-50991 |
| LabAssay ™ Cholesterol |
Wako |
Cat# 635-50981 |
| Oil red O stain kit |
Abcam |
Cat# ab150678 |
| iScript cDNA Synthesis Kit |
BIO-RAD |
Cat# 170-8891 |
| Sybr Green Master Mix |
Applied biosystems |
Cat# A25778 |
|
| Deposited data |
|
| Single cell RNA-seq |
This paper |
GSE148646 |
|
| Experimental models: Cell lines |
|
| Mouse: EO771 cells |
Gift from Rolf Brekken Lab, University of Texas Southwestern Medical Center |
N/A |
| Mouse: Met1 cells |
Gift from Rolf Brekken Lab, University of Texas Southwestern Medical Center |
N/A |
| Human: ZR-75-1 cells |
Hamon Cancer Center Collection, University of Texas Southwestern Medical Center |
N/A |
| Human: MDA-MB-231 cells |
Hamon Cancer Center Collection, University of Texas Southwestern Medical Center |
N/A |
| Human: MDA-MB-453 cells |
Hamon Cancer Center Collection, University of Texas Southwestern Medical Center |
N/A |
| Human: MCF7 cells |
Hamon Cancer Center Collection, University of Texas Southwestern Medical Center |
N/A |
|
| Experimental models: Organisms/strains |
|
| Mouse: Adipoq-rtTA |
Deng et al. (2018)
|
N/A |
| Mouse: aP2-MitoNEET |
Kusminski et al. (2012)
|
N/A |
| Mouse: TRE-Xbp1s |
Deng et al. (2018)
|
N/A |
| Mouse: Adipoq-Xbp1s |
Generated in-house |
N/A |
| Mouse: MMTV-PyMT |
Gift from Jeffrey Pollard Lab, Albert Einstein College of Medicine |
N/A |
| Mouse: Adipoq-Cre |
The Jackson Laboratory |
JAX: 028020; RRID: IMSR_JAX:028020 |
| Mouse: TRE-Cre |
The Jackson Laboratory |
JAX: 006234; RRID: IMSR_JAX:006234 |
| Mouse: Rosa26-mT/mG |
The Jackson Laboratory |
JAX: 007676; RRID: IMSR_JAX:007676 |
|
| Oligonucleotides |
|
| Primers for qPCR |
See Table S4
|
N/A |
|
| Software and algorithms |
|
| Image J |
NIH |
https://imagej.nih.gov/ij/
|
| Prism |
GraphPad |
https://www.graphpad.com/
|
| R studio |
RStudio |
https://www.rstudio.com/
|
| Cell Ranger |
10X GENOMICS |
https://www.10xgenomics.com/
|
| Seurat package 3.0 |
Satija Lab and Collaborators |
https://satijalab.org/seurat/
|
| MetaboAnalyst 5.0 |
MetaboAnalyst |
https://www.metaboanalyst.ca/
|
| DAVID 6.8 |
DAVID Bioinformatics Resources |
https://david.ncifcrf.gov/home.jsp
|
|
| Other |
|
| 60% HFD paste |
BioServ |
S1850 |
| Normal chow diet |
Lab Diet |
5058 |
| Doxycycline chow diet (600 mg/kg diet) |
BioServ |
S4107 |
