Figure 4.

The expression of GPER is modified by E2 treatment in human GB-derived cell lines and an astrocytoma cell line. (A) U251, (B) U87, (C) LN229, and (D) T98G cells were treated with E2 (1, 10 and 100 nM, and 1 µM) or V (cyclodextrin) for 24 h. The upper panels illustrate the representative western blots for GPER and α-tubulin. The lower panels demonstrate the corresponding results of the densitometric analysis. Results are expressed as the mean ± SEM, n=5 (*P<0.05 and **P<0.01). GPER, G-protein coupled estrogen receptor; V, vehicle.