Figure 5.

Neutralization activity induced by mRNA vaccination in SARS-CoV-2 naive and PI individuals vaccinated with a short or a long interval

(A and B) Neutralizing activity was measured by incubating pseudoviruses bearing SARS-CoV-2 S glycoproteins (D614G, Omicron, or BA.4/5), with serial dilutions of plasma for 1 h at 37°C before infecting 293T-ACE2 cells. Neutralization ID₅₀ values were determined using a normalized non-linear regression using GraphPad Prism software. D2: 1 (SI) or 3 (LI) weeks after the second dose, D3: 2 (SI) or 4 (LI) weeks after the third dose, and M3: 3 (SI) or 4 (LI) months after the third dose. Naive (A) and PI (B) donors vaccinated with the SI are represented by blue and yellow points, respectively, and naive (A) and PI (B) donors vaccinated with the LI are represented by red and black points, respectively. Undetectable measures are represented as white symbols, and limits of detection are plotted. Error bars indicate means ± SEM (^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ns, non-significant). For naive donors vaccinated with the SI, n = 45 at D2, n = 33 at D3, and n = 18 at M3. For naive donors vaccinated with the LI, n = 20 at D2, D3, and M3. For PI donors vaccinated with the SI, n = 16 at D2, n = 11 at D3, and n = 3 at M3. For PI donors vaccinated with the LI, n = 11 at D2, D3, and M3.