Fig. 4.

Both NOTCH3 and SMARCA4 can regulate the proliferation, invasion, migration, and apoptosis in colon cancer cells. (A, B) The CCK‐8 assay was performed to detect the proliferative activity of HT29 cells and HT115 cells in different groups (n = 3). (C–F) The effect of knocking down NOTCH3 or SMARCA4 on the ability of HT29 cells and HT115 cells' clone formation ability was detected by the clone formation assay and statistical analysis was performed (n = 3). (G–J) Transwell invasion assays were performed to detect the invasion abilities of HT29 cells and HT115 cells in different groups and the related statistical analysis was performed (scale bar: 100 μm; n = 3). (K–N) The scratch assays were performed to detect the migration abilities of HT29 cells and HT115 cells in different groups, and the related statistical analysis was performed (scale bar: 100 μm; n = 3). (O–R) Flow cytometry was performed to detect the apoptosis rate of HT29 cells and HT115 cells in different groups and its statistical analysis was performed (n = 3). Data are shown as presented as mean ± SEM. Statistical analyses were performed using two‐way ANOVA for CCK‐8 comparison in A and B. Multiple unpaired Student's t‐tests were using for the remaining data. *P < 0.05, **P < 0.01, ***P < 0.001; ns, no significant; NC, negative control; si, short interfering. [Colour figure can be viewed at wileyonlinelibrary.com]