TABLE 1.
E. coli strains used in this study
| Strain | Reference or source | Description or genotype |
|---|---|---|
| CC118 | 38 | araD139 Δ(ara-leu)7697 argE(Am) galE galK ΔlacX74 ΔphoA20 recA1 rpoB rpsE thi |
| DH5α | 54 | deoR endA1 gyrA96 hsdR17 φ80ΔlacZM15 recA1 relA1 supE44 thi-1 Δ(lacIZYA-argF)U169 |
| DH5αλpir | 41 | DH5α carrying λpir function necessary for autonomous replication of pCVD422-based suicide vector |
| E2348/69 | 35 | Prototype O127:H6 EPEC strain carrying pMAR2; NaIr |
| HB101 | 54 | ara-14 galK2 Δ(gpt-proA)62 hsdS20 lacY1 leuB6 mtl-1 recA13 rpsL20 supE44 thi-1 xyl-5 Δ(mcrC-mrr) (F′) |
| TG1 | 54 | ΔhsdS Δ(lac-proAB) supE thi F′ [traD36 proAB+ laclq ΔlacZM15] |
| TOP10 | Invitrogen | araD139 Δ(ara-leu)7697 deoR endA1 galK galU ΔlacX74 φ80ΔlacZM15 mcrA Δ(mrr-hsdRMS-mcrBC) nupG recA1 rpsL (F−) |
| UMD901 | 74 | E2348/69 bfpA (C129S) |
| UMD932 | 4 | E2348/69 bfpP::aphA-3 |
| UMD934 | This study | E2348/69 bfpE::aphA-3 |