TABLE 2.
Source plasmids used in this study
| Plasmid | Reference or source | Description |
|---|---|---|
| pBAD/myc-His B | Invitrogen | Expression vector with araBAD promoter, araC regulator, and 3′ double-epitope tag (Ap) |
| pBAD/gIII A | Invitrogen | Expression vector with araBAD promoter, araC regulator, 5′ phage fd gene III signal sequence, and 3′ double-epitope tag (Ap) |
| pCVD442 | 18 | Positive-selection suicide vector carrying sacB, oriR6K, and mobRP4 (Ap) |
| pCVD433::31 TnphoA | 16 | MluI fragment from EPEC mutant 31-6-1(1) containing bfpA4::TnphoA cloned into the MluI site of pCVD433 (Cm) |
| pHZZ4B1 | 75 | pACYC184 carrying a 4.0-kb BamHI fragment of pMAR2 that contains a partial bfp gene cluster (′bfpD bfpE bfpF bfpP bfpH′) (Cm) |
| pKDS135 | 60 | pBR322 carrying the entire bfp gene cluster |
| pMA632 | 1 | pBluescript II SK (+) carrying ′phoA-lacZα cassette |
| pMAR2 | 5 | Enterocyte adherence factor plasmid present in EPEC strain E2348/69 |
| pMD103 | 31 | pBluescript derivative carrying lacZYA |
| pRK2073 | 10 | Helper plasmid used to mobilize suicide vector |
| pTrc99A | 2 | Low-copy trc promoter expression vector carrying the lacIq gene (Ap) |
| pUC18K | 41 | pUC18 carrying a nonpolar aphA-3 kanamycin resistance cassette (Ap Kn) |