TABLE 3.

Oligonucleotide primers used in this study

Primer	Sequenceab	Annealing sitea	Restriction site(s)b
Donne-200	5′-GGAAGAACAGTATGTCGAGC-3′	aphA-3
Donne-211	5′-GGGGATCCATTACCTCAGCCCCAGAGCGGCT-3′	phoA	BamHI
Donne-222	5′-GATATTGCCGTGGTACGTT-3′	phoA
Donne-223	5′-AACGTACCACGGCAATATC-3′	phoA
Donne-224	5′-CGGGGTACCCCCGGGCCTGTTCTGGAAAACC-3′	phoA	KpnI, AvaI-XmaI
Donne-227	5′-GGGGATCCGGCCTGCCCGGTTATTATTA-3′	lacZ	BamHI
Donne-228	5′-ATAGGTACCCCCGGGCTGGCCGTCGTTTTACAA-3′	lacZ	KpnI, AvaI-XmaI
Donne-243	5′-ATAGTCCATGGCGAAAGAGAAATTAAACAGACTGC-3′	bfpE	NcoI
Donne-256	5′-TAATCCCGGGTGCTAGCTTACTCGAGGAATCTTGCTGCATCAC-3′	bfpE	AvaI-XmaI, NheI, XhoI
Donne-272	5′-CCCATCGCCAATCAGCAAAATA-3′	phoA
Donne-273	5′-CGGGCCTCTTCGCTATTA-3′	lacZ
Donne-305	5′-TAATCCCGGGTGCGCCTTTTACTTTCT-3′	bfpE	AvaI-XmaI
Donne-315	5′-TAACGCCGGCTCAGTTCAATGGCAGGATGAC-3′	bfpE	Ngo-MIV
Donne-316	5′-TAAACCCGGGAGCAACATTTTTCAGAACAGTCAT-3′	bfpE	AvaI-XmaI
Donne-317	5′-TAAACCCGGGAGCTATCGACCAGGGAATAAAT-3′	bfpE	AvaI-XmaI
Donne-318	5′-TTAACCCGGGAGCAAAGAAGAGACGCAACCT-3′	bfpE	AvaI-XmaI
Donne-319	5′-TAAACCCGGGAGCCCACCCCCTCATCGCTTCA-3′	bfpE	AvaI-XmaI
Donne-343	5′-TCACTGCCGGCGAAGCCTTACGAATAAT-3′	bfpE	NgoMIV
Donne-344	5′-CGGACATTTTTCACTGGTC-3′	phoA
Donne-383	5′-ACGCTGCGCGTAACCACCACA-3′	f1 origin
Donne-384	5′-ATATCCCGGGGCCCATTCGCCATTCAGG-3′	lacZ	AvaI-XmaI
Donne-385	5′-TATCGGGCCCCTGTTCTGGAAAACCGGGCTGCTCAG-3′	phoA	EcoO1091
Donne-386	5′-AATTGGGGCCCATTCGCCATTCAGG-3′	lacZ	EcoO1091
Donne-486	5′-TCAGGACCATGGCGGAAGCCTTACGAATAAT-3′	bfpE	NcoI
Donne-507	5′-CTGTTGCTCTAGAAATCTTGCTGCATCAC-3′	bfpE	XbaI
Donne-508	5′-GGCGTGCTCTAGAACATTTTTCAGAACAGTCAT-3′	bfpE	XbaI

^aThe underlined portion of the primer sequence anneals to the gene indicated. 

^bThe italicized portion of a primer sequence indicates a restriction site present in a PCR product prepared with that primer.