TABLE 4.
UPHLC-MS analysis tentative identification of the secondary metabolites from C. burhia root methanol extract (negative ionization mode).
| No. | RT (min) | Mass | Tentative identification | Chemical formula | Compound class | B. peak (m/z) |
| 1 | 7.794 | 432.1273 | Apiosylglucosyl 4-hydroxybenzoate | C18 H24 O12 | Glycoside | 431.1273 |
| 2 | 8.287 | 207.0894 | Phenylpropionylglycine | C11 H13 NO3 | Acyl glycine | 208.0894 |
| 3 | 8.49 | 462.1168 | Tricin 4′-apioside | C22 H22 O11 | Flavone | 461.1168 |
| 4 | 8.871 | 416.1103 | 3′,4′-Dihydroxyflavone 4′-glucoside | C21 H20 O9 | Flavone | 415.1103 |
| 5 | 9.213 | 372.1214 | 7,8,3′,4′,5′-Pentamethoxyflavone | C20 H20 O7 | flavone | 371.1214 |
| 6 | 9.351 | 286.0481 | 5,7,2′,3′-Tetrahydroxyflavone | C15 H10 O6 | Flavone | 285.0481 |
| 7 | 9.507 | 370.1056 | Neouralenol | C20 H18 O7 | Flavone | 369.1056 |
| 8 | 9.614 | 406.0905 | 5,6,3′,5′-Tetrahydroxy-3,7,8,4′-tetramethoxyflavone | C19 H18 O10 | Flavonoids | 405.0905 |
| 10 | 9.856 | 270.0536 | Demethyltexasin | C15 H10 O5 | Isoflavonoe | 269.0536 |
| 11 | 9.942 | 138.0316 | p-Salicylic acid | C7 H6 O3 | Phenol | 137.0316 |
| 13 | 10.034 | 300.0636 | Kaempferide | C16 H12 O6 | Flavone | 299.0636 |
| 14 | 10.25 | 200.1051 | Decenedioic acid | C10 H16 O4 | Phyto | 199.1051 |
| 15 | 10.358 | 584.2616 | Pubescenol | C32 H40 O10 | Withanolide | 583.2616 |
| 16 | 10.424 | 254.0584 | 7,4′-Dihydroxyflavone | C15 H10 O4 | Flavone | 253.0584 |
| 17 | 10.553 | 284.0683 | Texasin | C16 H12 O5 | Phyto | 283.0683 |
| 18 | 10.756 | 390.0955 | 5,7,2′-Trihydroxy-3,6,4′,5′-tetramethoxyflavone | C19 H18 O9 | Flavone | 389.0955 |
| 19 | 10.822 | 354.1103 | 2,3-Dehydrokievitone | C20 H18 O6 | Phyto | 353.1103 |
| 20 | 10.921 | 268.0373 | Coumestrol | C15 H8 O5 | Coumestans | 267.0373 |
| 21 | 11.214 | 454.1632 | 5,2′,4′,5′-Tetrahydroxy-3-(3-hydroxy-3-methylbutyl)-6″,6″ dimethylpyrano[2″,3″:7,8]flavone | C25 H26 O8 | Flavone | 453.1632 |
| 22 | 11.217 | 298.048 | 8-Methoxycoumestrol | C16 H10 O6 | Coumestans | 297.048 |
| 23 | 11.293 | 352.0607 | 3′-O-Methyl-(-)-epicatechin-5-O-sulfate | C16 H16 O7S | Flavonoids | 351.0607 |
| 24 | 11.448 | 624.2634 | Kanokoside D | C27 H44 O16 | Terpene glycoside | 623.2634 |
| 25 | 11.476 | 578.2573 | Withaperuvin H | C30 H42 O9 S | Withanolide | 577.2573 |
| 26 | 11.515 | 400.116 | Torosaflavone A | C21 H20 O8 | Flavonoids | 399.116 |
| 27 | 11.52 | 468.1045 | Gyrophoric acid | C24 H20 O10 | Phyto | 467.1045 |
| 28 | 11.561 | 330.241 | 5,8,12-trihydroxy-9-octadecenoic acid | C18 H34 O5 | Fatty acids | 329.241 |
| 29 | 11.63 | 352.0947 | Psoralidin oxide | C20 H16 O6 | Coumestans | 351.0947 |
| 30 | 11.787 | 314.0793 | Luteolin 5,3′-dimethyl ether | C17 H14 O6 | Flavonoids | 313.0793 |
| 31 | 12.099 | 256.0738 | 6-Demethylvignafuran | C15 H12 O4 | Isoflavonoid | 255.0738 |
| 32 | 12.141 | 562.2625 | 19-Hydroxycinnzeylanol 19-glucoside | C26 H42 O13 | Glycosides | 561.2625 |
| 33 | 12.638 | 354.1101 | 2,3-Dehydrokievitone | C20 H18 O6 | Flavanone | 353.1101 |
| 34 | 13.249 | 220.0737 | Polygonolide | C12 H12 O4 | Coumarins | 219.0737 |
| 35 | 13.368 | 322.1208 | 5,7-Dihydroxy-8-prenylflavone | C20 H18 O4 | Flavone | 321.1208 |
| 36 | 13.375 | 368.1228 | Aurmillone | C21 H20 O6 | Isoflavonoe | 367.1228 |
| 37 | 13.512 | 438.1681 | Morusignin L | C25 H26 O7 | Flavones | 437.1681 |
| 38 | 13.572 | 676.2315 | Artonin D | C40 H36 O10 | Chalcones | 675.2315 |
| 39 | 13.573 | 452.11 | Cinchonain Ib | C24 H20 O9 | Phyto | 451.11 |
| 40 | 13.581 | 338.1163 | (-)-Glyceollin I | C20 H18 O5 | Phytoalexins | 337.1163 |
| 41 | 13.991 | 336.1001 | Isosojagol | C20 H16 O5 | Coumestans | 335.1001 |
| 42 | 14.229 | 440.1835 | Exiguaflavanone C | C25 H28 O7 | Flavanone | 439.1835 |
| 43 | 14.474 | 354.1102 | 2,3-Dehydrokievitone | C20 H18 O6 | Flavanone | 353.1102 |
| 44 | 14.607 | 450.0928 | Exserohilone | C20 H22 N2 O6 S2 | Indoles | 449.0928 |
| 45 | 14.721 | 342.1104 | 5,7,2′,5′-tetramethoxyflavone | C19 H18 O6 | Flavone | 341.1104 |
| 46 | 14.82 | 334.0844 | Sophoracoumestan A | C20 H14 O5 | Coumeston | 333.0844 |
| 47 | 15.079 | 340.0952 | Methylophiopogonone A | C19 H16 O6 | Flavonoid | 339.0952 |
| 48 | 15.08 | 324.1364 | Isobavachalcone | C20 H20 O4 | Chalcones | 323.1364 |
| 49 | 15.283 | 390.1831 | Paratocarpin B | C25 H26 O4 | Chalcones | 389.1831 |
| 50 | 15.494 | 340.0946 | Methylophiopogonone A | C19 H16 O6 | Flavonoid | 339.0946 |
| 51 | 15.641 | 406.1783 | Honyucitrin | C25 H26 O5 | Flavanone | 405.1783 |
| 52 | 17.708 | 296.2354 | 12-oxo-10Z-octadecenoic acid | C18 H32 O3 | Fatty acids | 295.2354 |
| 53 | 18.511 | 272.2355 | 2-Hydroxyhexadecanoic acid | C16 H32 O3 | Fatty acids | 271.2355 |
RT, retention time; B. Peak, base beak.