Fig. 2.

Analysis of the specific humoral responses. The anti-L1, A27, A33 and B5 antibody levels were determined by ELISA using purified IHD-J VV as the plate-bound antigen. The bars indicate the average of the values of the animals of each group. The responses were compared among the groups, and the mice of G3, G4, and G5 showed significant increases in specific antibody titres at T2 and T3. Statistical differences using one-way ANOVA parametric tests and Bonferroni/Newman-Keuls analysis of variance are shown: (^**) p < 0.01; (^***) p < 0.001.