Fig. 3.

Nigericin does not inhibit vaccinia virus entry. (A) HeLa cells in 6-well plates were pretreated with 2 μM nigericin for 1 h. Cells were then infected with vaccinia virus at an MOI of 0.1 in the presence of nigericin. At 1 hpi, the media containing the virus and nigericin was removed and the cells were washed three times with fresh growth media. Growth media without nigericin was then added to the cells and virus titres were determined at 5 and 24 hpi by plaque assays on BHK21 cells. (B) HeLa cells in 6-well plates were infected with vaccinia virus at an MOI of 0.1 in the absence of nigericin. At 4 hpi, the cells were left untreated or were treated with 2 μM nigericin. Virus titres were determined at 5 and 24 hpi by plaque assays on BHK21 cells. All results are representative of three independent experiments. Error bars represent the standard error of the mean.