FIGURE 6.

The genus Lactobacillus-mediated generation of 5-MIAA improved NAFLD. (A–C) Serum TC, TG, and LDL-C in NAFLD mice treated with vehicle or 5-MIAA (2.8 mM/kg). (D,E) Hepatic ALT and AST treated with vehicle or 5-MIAA (2.8 mM/kg). (F) H&E-stained sections and Oil red O staining of liver tissue of the CON, HFD, and 5-MIAA groups. (G–I) Hepatic SOD, MDA, and GSH. (J–L) Relative mRNA expression of GCLC, NQO1, and TXR1. (M) Western blot analyses of Keap1, Nrf2, NQO1, and HO-1. All the data were presented as the means ± SD from n = 6-8 mice per group. Significant differences compared with the CON group were indicated by asterisks: ^▲P < 0.05, ^▲▲P < 0.01, ^▲▲▲P < 0.001. Significant differences compared with the HFD group were indicated by asterisks: *P < 0.05, **P < 0.01, and ***P < 0.001.