Table 1.
Protection against a lethal ECTV challenge in A-strain mice vaccinated with Dryvax (DVX) in conjunction with BCV or vehicle (Anti-Veh).a At T = 0 days, A-strain mice were vaccinated with decreasing doses of Dryvax (DVX)c and administered BCV via oral gavage starting at T = 0 days (10 mg/kg) followed by 2.5 mg/kg doses on T = 2, 4, 6, 8, 10, 12, and 14 days. At T = 50 days, mice were challenged via the IN route with 20 PFU (10× LD50) of ECTV.
| Group | Cage | # of mice | DVXb dilution | BCVb | ECTV (T = 50 days) | Day of death (rtc)b | MTD ± SEMb | Mortality (%) |
|---|---|---|---|---|---|---|---|---|
| 1 | 1 | 10 | N/A | N/A | − | 0 | ||
| 2 |
2 | 10 | Veh |
Veh | + |
8,8,8,8,8,8,8,9,9,9 | 8.3 ± 0.2 | 100 |
| 3 | 10 | + | 8,8,8,8,8,9,9,9,9,9 | 8.5 ± 0.2 | 100 | |||
| 3 |
4 | 10 | Neatc |
Veh | + |
0∗ | ||
| 5 | 10 | + | 10 | 10 | 10∗ | |||
| 4 |
6 | 10 | 1:5c |
Veh | + |
0∗ | ||
| 7 | 10 | + | 0∗ | |||||
| 5 |
8 | 10 | 1:25c |
Veh | + |
0∗ | ||
| 9 | 10 | + | 0∗ | |||||
| 6 |
10 | 10 | 1:125c |
Veh | + |
0∗ | ||
| 11 | 10 | + | 0∗ | |||||
| 7 | 12 | 10 | 1:625c | Veh | + | 12 | 12 | 10∗ |
| 13 | 10 | + | 9,9,10 | 9.3 ± 0.3∗ | 30∗ | |||
P < 0.05 compared to controls.
Protocol is described in the legend of Fig. 2.
DVX, Dryvax; BCV, Brincidofovir; MTD, mean time to death; SEM, standard error of mean; rtc, relative to challenge.
PFU/mouse dilutions are 2.5 × 105 (for Neat), 5 × 104 (for 1:5), 1 × 104 (for 1:25), 2 × 103 (for 1:125), and 400 for (1:625).