TABLE 2.
Oligonucleotides used for gel shift assays
| Gene | SOS box label | Sequence of oligonucleotidea |
|---|---|---|
| recA | recA0 | tgaatcGAACaggtGTTCggctac |
| lexA | lexA1a | cctgtcGAACacatGTTtgattct |
| lexA1b | atatccGAACatttGaTCgaagcg | |
| lexA2a | cggttgGAgCcggacTTCcggcgc | |
| lexA2b | gtaatcGctCgcgtGTTCgacact | |
| ruvA | ruvA0 | gctatcGAACgggtGTTCtctcag |
| ruvC | ruvC0 | cgtatcGAACgattGTTCggaaat |
| ruvC2a | atttaccAtCgcacGTTCcatagg | |
| ruvC2b | gtgtgcGAtCgagcGTTtcccgaa | |
| ssb | ssb2a | gaactcGAcCgccaGcTCagcctc |
| ssb2b | ccatacGAAatcatGgTCatcctc | |
| dnaB | dnaB1 | gttgtcGAAtatgcGTTCgggtgc |
| recN | recN2a | cggctgGtgCgcaaGTTCcggttg |
| recN2b | catcgtcAACcgggGTTgggcgct | |
| dinP | dinP2a | ccgctgGAcCgcctGaTCgcattc |
| dinP2b | gcaaagGcACcttgtTTCgccgct | |
| recC | recC1 | gaagccGcACgagaGTTCgccggt |
| recC2a | cgacaaGAAggcccGTaCcgactg | |
| recC2b | gggataGcAgccgaGTTCgggctg |
a
In each case, the complementary oligonucleotide was annealed with the sequence given and the resulting double-stranded probe was used in the gel shift; the bases matching the consensus SOS box sequence are shown in uppercase.