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TABLE 3.

PCR primers

PCR product Sequence
Forward primer Reverse primer Annealing temp (°C) Length of product (bp)
recA-up GATCTAGACCAGGCTAGCGGTGTTGAG GACTAGTAACCTTTGCCGTAACTCTTC 58 355
ssb-up1 ACGGCGGAAAAGTCGAAAAGGTG GCCGCCTCCCGCCAGATA 58 488
ssb-up2 GGCCGCACACGACCACAG GAGCCTACGTAACCGCACCGACAG 58 376
ssb-up3 GCCGTTCGCGACACTGACATT TCTCCAAGGACGGGGCTAG 55 272
uvrA-up1 ATCGTGGCGCCGGGCAGGAAGC GGATAACCCGGTGAAGACGAT 55 347
uvrA-up2 CTGTCGCCGTGGGTGAGCA AGCAGTGGCGCATATGACAACAGT 55 381