TABLE 3.
PCR product | Sequence
|
|||
---|---|---|---|---|
Forward primer | Reverse primer | Annealing temp (°C) | Length of product (bp) | |
recA-up | GATCTAGACCAGGCTAGCGGTGTTGAG | GACTAGTAACCTTTGCCGTAACTCTTC | 58 | 355 |
ssb-up1 | ACGGCGGAAAAGTCGAAAAGGTG | GCCGCCTCCCGCCAGATA | 58 | 488 |
ssb-up2 | GGCCGCACACGACCACAG | GAGCCTACGTAACCGCACCGACAG | 58 | 376 |
ssb-up3 | GCCGTTCGCGACACTGACATT | TCTCCAAGGACGGGGCTAG | 55 | 272 |
uvrA-up1 | ATCGTGGCGCCGGGCAGGAAGC | GGATAACCCGGTGAAGACGAT | 55 | 347 |
uvrA-up2 | CTGTCGCCGTGGGTGAGCA | AGCAGTGGCGCATATGACAACAGT | 55 | 381 |