Table 2.

Oligonucleotide primers used in MPCR

Virus	Genomic region (ORF)	Primer sequence	Length of amplicon (bp)
VACV	F4L (genus-specific region)	5′-cgttggaaaacgtgagtccgg-3′	292
		5′ attggcgttttttgcagccag-3′
CPXV	B9R	5′-atcagatggaattatctctcacccg-3′	421
		5′ gataatttgatccatctcgtccacc-3′
MPXV	E5R	5′-atgttgatattaataatcgtattgtggtt-3′	581 (West African)
		5′-aaagtcaatacactcttaaagattctcaa-3′	832 (Central African)
VARV	B11R-B12R	5′-catccgatattattgtaaccacaatg-3′	203
VACV	C9L	5′-ggtgtagtcgtaatcgtaatcgtctaatt-3′ 5′-aagatactctatgatagttgtaaaacatttaacatc-3′	492
		5′-cccaacatttctaaatctcctcgt-3′

ORF, open reading frame; ORF names are given according to the designations conventionally accepted for each virus (see Fig. 1). VACV, vaccinia virus strain Copenhagen [29]; CPXV, cowpox virus strain GRI-90 [28]; MPXV, monkeypox virus strain Zaire-96-I-16 [27]; VARV, variola virus strain India-1967 [24].