Table 1.
Comparison of clinical signs presentation during Phase 1 of the study, on CAST/EiJ mice infected with different doses of Variola virus (animals challenged at 8–9 weeks of age).
| Dosage | 5 × 102* | 5 × 103* | 5 × 104* | 5 × 105* | 5 × 106* |
|---|---|---|---|---|---|
| Decreased activity onset (number of animals) | Day 9 pi (2/5) | Day 7 pi (4/5) | Day 7 pi (5/5) | Day 5 pi (5/5) | Day 5 pi (4/5) |
| Reduce grooming onset (number of animals) | Day 7 pi (5/5) | Day 7 pi (4/5) | Day 7 pi (5/5) | Day 7 pi (5/5) | Day 7 pi (5/5) |
| Oral/nasal erythema (number of animals) | NO | NO | Day 7 pi (2/5) | Day 7 pi (2/5) | Day 7 pi (5/5) |
| Ocular discharge (number of animals) | NO | Day 10 pi (1/5) | Day 7 pi (2/5) | Day 7 pi (3/5) | Day 7 pi (3/5) |
| Rear paw/tail skin lesions (number of animals) | NO | Day 10 pi (2/5) NP | Day 12 pi (2/5) NP | Day 12 pi (2/5) | Day 12 pi (3/5) NP |
| Complete recovery** | Day 14 pi | Day 17 pi | Day 17 pi | Day 19 pi | Day 19 pi |
Mice from gamma irradiated VARV and vehicle (PBS + .05% BSA) groups did not show any clinical signs mentioned above.
pi = days post inoculation.
NO = Never observed.
NP = Negative by PCR.
*
Challenged groups, in plaque forming units (pfu).
**
Pain score back to 0 points.