Table 3.

Immune response of 8–9 weeks old CAST/EiJ mice during Phase 1, after challenge with different doses of Variola virus.

ID #	Group*	Age	Serum˟	Positive Dilutionˠ
17	Diluent	9 weeks old	21 days pi	BLD
18	Diluent	9 weeks old	21 days pi	BLD
28	GAMIR	8 weeks old	21 days pi	BLD
29	GAMIR	8 weeks old	21 days pi	BLD
30	GAMIR	8 weeks old	21 days pi	1:50
31	5 × 102	8 weeks old	21 days pi	>1:3200
32	5 × 102	8 weeks old	21 days pi	>1:3200
11	5 × 102	9 weeks old	21 days pi	1:50
12	5 × 102	9 weeks old	21 days pi	>1:3200
13	5 × 102	9 weeks old	21 days pi	1:50
26	5 × 103	8 weeks old	21 days pi	>1:3200
27	5 × 103	8 weeks old	21 days pi	>1:3200
14	5 × 103	9 weeks old	21 days pi	>1:3200
15	5 × 103	9 weeks old	21 days pi	>1:3200
16	5 × 103	9 weeks old	21 days pi	>1:3200
21	5 × 104	8 weeks old	21 days pi	>1:3200
22	5 × 104	8 weeks old	21 days pi	>1:3200
23	5 × 104	9 weeks old	21 days pi	>1:3200
24	5 × 104	9 weeks old	21 days pi	>1:3200
25	5 × 104	9 weeks old	21 days pi	>1:3200
4	5 × 105	9 weeks old	21 days pi	>1:3200
5	5 × 105	9 weeks old	21 days pi	>1:3200
6	5 × 105	8 weeks old	21 days pi	>1:3200
7	5 × 105	8 weeks old	21 days pi	>1:3200
8	5 × 105	8 weeks old	21 days pi	>1:3200
1	5 × 106	9 weeks old	21 days pi	>1:3200
2	5 × 106	9 weeks old	21 days pi	>1:3200
3	5 × 106	9 weeks old	21 days pi	>1:3200
19	5 × 106	8 weeks old	21 days pi	>1:3200
20	5 × 106	8 weeks old	21 days pi	>1:3200

BLD = below limit of detection.

*Challenged groups, in plaque forming units (pfu).

*Diluent = PBS + .05%BSA. GAMIR = gamma irradiated Variola virus.

^˟pi = days post inoculation, day of sample collection.

^ˠSera were tested at 1:50, 1:200, 1:800 and 1:3200 dilutions.