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. 2022 Oct 5;610(7931):381–388. doi: 10.1038/s41586-022-05282-z

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© The Author(s), under exclusive licence to Springer Nature Limited 2022, corrected publication 20232023Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Extended Data Fig. 8 — (a,c,e) H3K9me3 (a), H3K27me3 (c) or H3K9ac (e) staining of A549^ACE cells 24 h after SARS-CoV-2^WT, SARS-CoV-2^ΔOrf8, or mock infection at MOI = 1. (b,d,f) Quantification of H3K9me3 (b), H3K27me3 (d) or H3K9ac (f). For H3K9me3 N = 39 (SARS-CoV-2), 35 (SARS-CoV-2^ΔOrf8), for H3K27me3 N = 48 (SARS-CoV-2), 37 (SARS-CoV-2^ΔOrf8), for H3K9ac N = 94 (SARS-CoV-2), 120 (SARS-CoV-2^ΔOrf8) cells normalized to uninfected neighbor cells from 1-2 independent infections. Dotted line indicates relative signal in Mock infected condition. (g) Quantification of H3K9me3 in infected cells and neighboring cells from the same tissue slice for each individual patient sample shown separately, relative to control samples. For sample 1, N = 3 infected and 48 uninfected, sample 2, N = 7 infected and 125 uninfected, sample 3, N = 5 infected and 55 uninfected cells. (b,d,f) 1-way ANOVA with post-hoc 2-sided t-test and Bonferroni correction. Scale bar = 10μM. Box plots centered on median, bounds at 25^th and 75^th percentile, minimum and maximum defined as median ± 1.5x interquartile range, with whiskers extended to lowest/highest value in range.

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