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Purification of soluble Fe(III) reductase
| Purification step | Amt of protein (mg) | Activity (U) | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Crude extract | 493.8 | 54.8 | 0.11 | 100 | 1 |
| Q-Sepharose | 38.4 | 37.8 | 0.98 | 69 | 9 |
| Butyl-Sepharose | 5.4 | 21.3 | 3.94 | 39 | 36 |
| Superdex 200 | 0.5 | 15.7 | 31.40 | 29 | 282 |
| MonoQ | 0.17 | 11.1 | 65.30 | 20 | 594 |
