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Detection limit of multiplex real-time PCR with dried reagents. Variola J7R DNA clone was serially diluted from 105 to 0.1 fg per reaction. Each curve represents the average fluorescence value of three replicates. Both FAM and TET probes signals were detected as expected in the presence of variola DNA fragment. The assay has a detection limit of 0.1 fg per reaction (25 copies).
