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Detection limit of multiplex real-time PCR with dried reagents. Monkeypox virus genomic DNA was diluted from 106 to 10 fg per reaction. Each curve represents the average fluorescence value of two to three replicates. Only TET signal was detected in the presence of monkeypox DNA. FAM signal was absent because it is specific for Variola. The assay has a detection limit of 10 fg per reaction (approximately 50 copies).
