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Standard curves were generated by plotting PCR threshold cycles (Ct) against log concentration of J7R cloned DNA. Regression analysis showed a broad dynamic range that with seven orders of magnitude for both FAM and TET reporters. (a) Dried PCR reagents; (b) wet PCR reagents; (c) regression analysis curves generated by plotting PCR threshold cycles (Ct) against log fg of monkeypox virus genomic DNA obtained with wet and dried PCR reagents. The coefficients of correlation are indicated.
