Table 5.

Presence of OPV Virulence Ortholog family members in monkeypox and variola viruses

^aSee footnote b of Table 4.

^bWe removed 29 fragmented ORFs that were previously annotated in VARV-BSH-75 genome, including A26L, A27L, A28L, A29L, A39L, A40R, A42R, A43R, A47L, C1L, C7L, D17L, D16L, D13L, D10L, D9L, D8L, D1L, B20R, B19R, B14L, B11R, B7R, B4L, B3L, B2L, E7L, O3L, and J6R. The reannotated VARV-BSH genome contains 162 ORFs. Our analysis suggests the VARV-BSH ORF D3L was likely not functional thus this ORFs was omitted from our reannotation. The original annotation was described by Massung et al. (1994). These updated annotations are available from the POCsdb (http://athena.bioc.uvic.ca).

^cFragment.

^dVCP-MPXV has a single nucleotide deletion leading to a stop codon that terminates the protein 13 aa into the fourth CCP module and 43 aa from the C terminus.

^eGene is missing.