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. 2001 Aug;183(15):4484–4492. doi: 10.1128/JB.183.15.4484-4492.2001

FIG. 5.

FIG. 5

Extracellular expression of AcbD in S. lividans TK23. The lanes of the gel (0.1% SDS–8% polyacrylamide) contained samples of lyophilized protein from 500 μl of dialyzed culture supernatant from each of the following strains (for details of conditions, see Materials and Methods): lane 1, S. lividans TK23/pUWL201 (cultured in TSB medium); lane 2, S. lividans TK23/pAS9 (cultured in TSB medium); lane 3, Actinoplanes sp. strain SE50/110 (cultured in acarbose production medium); left lane, molecular weight marker. The location of the AcbD band is indicated.