FIG. 7.

Comparison of catalytic mechanisms of α-amylase, CGTase, and ATase in the presence of acarbose alone or together with d-glucose (Glc) or maltooligodextrins (Mal_n) as transglycosylation donors or acceptors. The location of the substrate or inhibitor product, respectively, relative to the catalytic site (cat.) is indicated. The plain or crossed arrows indicate the ability or inability, respectively, to split the glycosidic bonds. The enzymes and data are as follows: PPA11, porcine pancreatic amylase II (10, 18); CGTase, CGTase from B. circulans 251 (28); ATase, AcbD, ATase (This study).